Journal: STAR Protocols
Article Title: Protocol for generating and using human iPSC-derived microglia-containing air-liquid-interface cortical organoid cultures
doi: 10.1016/j.xpro.2025.103915
Figure Lengend Snippet: Electrophysiological recording of MG-ALI-CO (A) Schematic overview of the different steps preceding and following electrophysiological recording. Created with BioRender.com. (B) MG-ALI-CO bright-field image as seen from the electrophysiology setup microscope. Scale bar, 50 μm. (C) Representative trace of an individual neuron’s electrophysiological recording. (D) Quantification of sEPSC amplitude and frequency from individual neurons in DIV120-170 MG-ALI-CO (6 weeks after MacPre integration; 10 5 integrated MacPre). Data are represented as means + SEM, N=12 cells (1 cell = 1 MG-ALI-CO). (E) MG-ALI-CO immunostained for patched neurons (biocytin) and IBA1. DAPI stains nuclei. Boxed areas are shown at higher magnification in the two right panels and show the close proximity of biocytin-filled neurons and microglia. Scale bars, 50 μm/ 10 μm.
Article Snippet: Upright Microscope (electrophysiology) , , SliceScope Pro 6000 (Scientifica).
Techniques: Microscopy